Abstract

Enzymes leading to the methylation of homocysteine to produce L-methionine in the mutant Corynebacterium glutamicum X300 were
investigated in this present study. S-adenosyl methionine served as a methyl group donor to homocysteine to form L-methionine. The
enzymatic pathway examined in this present study was cobalamine-independent pathway. No methylcobalamin homocysteine
transmethylase activity was detected in this microorganism.

Key words: S-adenosylmethionine; methyl group donor; homocysteine; L-methionine; methylcobalamin homocysteine transmethylase