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Original Article

J App Pharm Sci. 2019; 9(12): 103-107


Effects of progesterone and estradiol on the inflammatory and apoptotic markers of ovariectomized rats challenged with acute septic systemic inflammation

Sinan Subhi Farhan, Samir Saad Mahgoub, Saad Abdulrahman Hussain.




Abstract
Cited by 1 Articles

The inflammatory responses during a septic systemic inflammation were affected by the differential role of progesterone and estrogen that demonstrated pro-inflammatory and anti-inflammatory roles. The present study was designed to evaluate the differential effects of estradiol and progesterone supplementation on the inflammatory and apoptotic responses in an ovariectomized rat model of acute systemic septic inflammation (SSI). The present study was conducted on 60 female Wistar rats. 40 mg/kg estradiol and 5 mg/kg progesterone were given s.c. to ovariectomized (OVX) rats, after induction of systemic septic inflammation (SSI) through caecum puncture with a 21-gauge needle. Serum levels of TNF-α, CRP, ALT, estradiol, and progesterone were evaluated; additionally, iNOS, COX-II, and caspase-3 were evacuated in liver tissue homogenates using ELISA method. In OVX rats challenged with SSI, serum TNF-α, CRP and ALT levels were significantly increased associated with a decrease in serum estradiol levels. They also showed overexpression of iNOS and increased activity of COX-II and caspase-3 in the liver compared to non-OVX rats subjected to SSI. Supplementation with estradiol significantly decreases all serum and liver tissue markers of inflammation and decreased apoptosis. In contrast, in OVX rats supplemented with progesterone, SSI resulted in a significant increase of the studied markers. In conclusion, supplementation of estradiol in OVX rats challenged with SSI significantly attenuated the systemic and liver inflammatory and apoptotic markers. Meanwhile, supplementation with progesterone exacerbates the effects of the inflammatory markers and increases the tendency of apoptosis in the liver tissue.

Key words: septic systemic inflammation, liver, estradiol, progesterone, apoptosis






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