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Original Article

JVS. 2019; 5(1): 1-10
doi: 556


Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017

Akram Ibrahim Aboelkhair, Ayman Hany El-Deeb, Momtaz Abdelhady Shahin, Hussein Ali Hussein.


Abstract

Lumpy skin disease is an infectious viral disease caused by Lumpy skin disease virus (LSDV) affecting cattle causing skin lesions. It`s a member of Poxviridea family, genus Capri-pox, and closely related to sheep and goat pox. In Egypt, the protection from lumpy skin disease (LSD) among cattle population was carried out using sheep poxvirus vaccine. last year ( 2016-2017) revealed many cases of LSD in sheep pox vaccinated cattle in Egypt . In this study LSDV was isolated from sheep pox vaccinated cattle showing LSD signs to confirm LSD viral infection even in sheep pox vaccinated cattle . 130 Nodular samples were collected from 150 vaccinated cattle showing signs of LSD,Then Molecular detection of LSDV with PCR using G-protein-coupled chemokine receptor (G-PCR) gene primer revealed positive result for LSDV, So classical Isolation of LSDV was started by inoculation of nodules on Chorioallantoic membrane (CAM) 9-11 day old SPF eggs, then adaptation on Madin-Derby bovine kidney cell line ( MDBK ); confirmation of LSD isolated virus was done by using Electron microscopy (EM) on suspension of cell culture passage of the virus, Serological identification was done by serum neutralization test ( SNT ) using reference antisera which confirm presence of LSDV . By nucleotide sequences and genetic characterization was conducted on (G-PCR) gene segment of these isolates.

Key words: LSDV; MDBK; CAM; SNT; IFAT; SPV; PCR; Egypt






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