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Antibacterial effects of ER: YAG-PIPS, ER, CR: YSGG laser and conventional irrigation on enterococcus faecalis and candida albicans

Mehtap Zorlu Golge, Ugur Aydin, Ayse Buyuktas Manay.




Abstract
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Aim: Root canal treatment aims to disinfect root canal system from microorganisms. The limitations of contemporary disinfectants require investigation of new techniques. The aim of the present study is to compare the antibacterial efficiency of Er,Cr:YSGG [RFT] and Er:YAG [PIPS] lasers on Enterococcus faecalis [E. faecalis] and Candida albicans [C. albicans] to that of NaOCl.
Material and Methods: The present study included 140 single rooted human mandibular premolars. After preparing and sterilizing, the samples were divided into two groups. Root canals in group 1 were contaminated with E. faecalis while root canals in group 2 were contaminated with C. albicans. The root canal of each sample except negative control group was inoculated with 105cfu/ml of microorganisms in order to achieve standardization. Following incubation, all samples were randomly divided into 10 subgroups; Group 1A: Er,Cr:YSGG laser group. Group 1B: Er:YAG-PIPS laser. Group 1C: 5% NaOCl. Group 1D: positive control group. Group 1E: negative control group. Group 2A: Er,Cr:YSGG laser group. Group 2B: Er:YAG laser. Group 2C: 5% NaOCl. Group 2D: positive control group. Group 2E: negative control group. After disinfection process, the culture of each sample was tested to determine cfu/ml values for before incubation and after 4 week incubation.
Results: : Er:YAG and NaOCl groups lead to significant microorganism reduction [p0.05].
Conclusion: The use of Er:YAG-PIPS laser with NaOCl increased the success rate in the elimination of E.faecalis and C.albicanss

Key words: Candida albicans; Er,Cr:YSGG laser; Er:YAG-PIPS laser; enterococcus faecalis; NaOCl






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