The present study investigates the zoo-chemical profiling, anti-inflammatory and radical scavenging activity of Luffariella herdmani marine sponge extract. The sponge was collected from Unawatuna, Sri Lanka and extracted with methanol/dichloromethane (1:1 v/v) to prepare the Sponge Crude Extract (SCE). The SCE was used to screen the zoo-chemicals while anti-inflammatory activity was investigated by protein denaturation assay. Radical scavenging activity of the SCE was tested against 2, 2-diphenyl-1-picrylhydrazy (DPPH), nitric oxide (NO) and peroxide radicals while in vivo toxicity was evaluated by Artemia salina lethality assay. The results indicated the presence of terpenoids, alkaloids, anthraquinones, unsaturated sterols, sterols and saponins in the SCE while flavanoids, quinones, tannins and phenols were not reported. In vitro anti-inflammatory activity against protein denaturation with IC50 of 58.54 µg/ml was evidenced while radical scavenging activity was not reported against the tested radicals; DPPH, NO and peroxide. The SCE specified toxicity against A. salina larvae with LC50 of 14.34 µg/ml. The present results confirmed the presence of zoo-chemicals, in vitro anti-inflammatory and in vivo cytotoxic properties of L. herdmani marine sponge extract.
Key words: Anti-inflammatory, Artemia salina toxicity, Luffariella herdmani, Marine sponges, Radical scavenging
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