Home|Journals|Articles by Year|Audio Abstracts

Original Article

J App Pharm Sci. 2021; 11(7): 127-131

Method validation for the simultaneous estimation of three-bioactive components in combined extracts of three hepatoprotective plants using RP-HPLC method

Sharuti Mehta, Anil Kumar Sharma, Rajesh Kumar Singh.


Picrorhiza kurroa Royle ex Benth. (Scrophulariaceae), Andrographis paniculata (Burm.f.) Nees (Acanthaceae), Silybum marianum (L.) Gaernt (Sm). (Asteraceae) are well-known plants to treat various ailments. Simultaneous quantification and determination of picroside-I, andrographolide, and silybin were conducted in the combined extract of their respective plants, by reverse-phase high performance liquid chromatography (RP-HPLC). Analytical determination of the proposed HPLC method was validated as per International Conference for Harmonization guidelines. Determination and method validation was carried on RP-HPLC column. Picroside-I, andrographolide, and silybin were quantified as 1.232 ± 0.0102, 0.572 ± 0.0734, 2.037 ± 0.0635 µg/mg, respectively. Validated method for simultaneous estimation of analytes was investigated to be linear, specific, reproducible, and accurate. Recovery of identified analytes was within the specified limit of 99.65%–102.59%. The study reveals that the developed HPLC method exhibits potential for determination and quantification of picroside-I, andrographolide, and silybin in in combined extract of P. kurroa, A. paniculata, and S. marianum.

Key words: diterpeniod lactone, flavanolignan, high performance liquid chromatography, Kalmegh, Kutki, Milk Thistle

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Review(er)s Central
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.