A total of 100 samples were collected from patients suffering from urinary tract infections were admitted
and visited Maternity and Children Hospital in Al-Hilla city, during a period of three months from
September to November 2021. To confirm the identification of E. coli by use selective media,
biochemical tests, Api20E system and automated Vitek 2 system, it was found that, 33 isolates were
showed positive and identified as E. coli. Molecular detection of some virulence factors genes were
studied, out of 33 E. coli isolates, fimH gene was detected in 27(81.8%) isolates by the presence of (508
bp), pap gene was detected in 26(78.8%) isolates by the presence of (336bp), hlyA gene was detected in
10(30.3%) isolates by the presence of (1177bp), feoB gene was detected in 19(57.6%) isolates by the
presence of (470bp), usp gene was detected in 21(63.6%%) isolates by the presence of (440bp) and
24(72.7%) have iroN gene by the presence of (665bp) compared with allelic ladder.
Key words: Genes, PCR, E. coli, Urinary tract infections
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