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The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia SamplesNguyen Thanh Hoa, Nguyen Khang Son, Tran-Thi Phuong Hoa, Mai-Thi Giang, Le Ngoc Dung, Nguyen Minh Duc, Nguyen Manh Ha. Abstract | | | | Background: Sperm preparation is an important step during assisted reproduction, and different assisted reproductive techniques have different sperm quality requirements. For intrauterine insemination (IUI), the total motile sperm count is a predictor of a patient’s fertility. Objective: The aim of this study was to compare the sperm recovery rate and DNA fragmentation index (DFI) outcomes following density mini-gradient and single-layer centrifugation in preparation for intrauterine insemination (IUI). Methods: A total of 30 semen samples with concentrations under 15 million cells/ml were obtained, and each sample was divided into 3 aliquots, with each aliquot subjected to 1 of 3 separation methods: mini-gradient, single-layer using a 90% density layer (single 90-layer), and single-layer using a 45% density layer (single 45-layer). Total sperm motility and sperm recovery rates were compared before and after preparation using each method. Results: The sperm concentration obtained using single 45-layer was higher than the other groups (p
Key words: Sperm preparation, IUI, mini-gradient, single-layer centrifugation
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