The sponge Aaptos sp. can be developed and utilized as a new antioxidant source. This study aimed to investigate the antioxidant activity and acute toxicity of acetone extract of Aaptos sp. (ASE) and its compounds. Aaptos sp. was extracted by acetone then fractionated using vacuum liquid chromatography (VLC) and radial chromatography (RC) with each step intervened with thin layer chromatography (TLC). Compounds was identified by their physical properties, 1H, and 13C NMR spectrum. Antioxidant activity was assayed qualitatively and quantitatively toward the DPPH radical. Acute toxicity was assayed using brine shrimp lethality test (BSLT). Five compounds (AS1-AS5) were isolated, among which, two were succesfully identified as cholestanol and uracil. The qualitative DPPH scavenging showed antioxidant activity by the ASE, AS1, AS2, and AS, of these, only ASE has an IC50 value of 16.10 µg/mL. The ASE, AS1, AS2, and AS5 showed acute toxicity with LC50 values of 1041.50 µg/mL, 1488.33 µg/mL, 681.87 µg/mL, and 783.21 µg/mL, respectively. The study concluded the potential of Aaptos sp. for the development of antioxidants from marine sponge and could provide a variety of metabolites with less toxicity.
Key words: Aaptos sp., marine sponge, secondary metabolite, antioxidant, acute toxicity
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