High polyphenol content of cocoa pod husc extract causing it potential to be developed as antioxidant and tyrosinase inhibitory agent in cosmetic preparations. Phytosome system known could enhance skin penetration of phytoconstituent like polyphenol-rich extract. The objectives of this research were to formulate phytosome containing cocoa pod extract, develop phytosome complex into face serum preparation, and determine antioxidant and tyrosinase inhibitory activities of the extract and the formulated serum. The cocoa pod extract was developed into phytosome system by thin layer method using soy-phosphatidylcholine. The phytosome then develop into face serum formulation using viscolam mac 10 as gelling agent. The antioxidant activity was conducted by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay and tyrosinase inhibitory activity was conducted by colorimetric enzymatic assay. The cocoa pod extract has very strong antioxidant activity with inhibitory concentration (IC50) was 17.21 ppm. The extract also has tyrosinase inhibitory activity with IC50 was 199.98 ppm. The phytosome complex containing cocoa pod extract and phosphatidylcholine (1:1) has good entrapment efficiency (90.5%) with average particle size 672 nm . The formulated face serum has good physical characteristic and also has antioxidant and tyrosinase inhibitory activities that equal with marketed product (Hadalabo ultimate whitening milk, Rohto, Indonesia).
Key words: cocoa pod husc, phytosome, serum, inhibitor tyrosinase, antioxidant
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