Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Research

Evaluation of Genetic Diversity at GDF9 and BMP4 Gene by Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) in Indian Goats

Vaishali Khare,Shrikant Joshi,Mohan Singh Thakur,Gandhi Prasad Lakhani,Ajit Pratap Singh.

Genetic diversity of three Indian goat breeds namely Barbari, Sirohi and Black Bengal goats (Capra hircus) was evaluated using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) method to detect polymorphism in two candidate genes (GDF9 and BMP4) in a sample of 90 goats with 30 goats of each breed. Both of the studied loci were polymorphic, having three variants for GDF9 and two variants for BMP4, in the studied population. Polymorphic information content (PIC) criterion revealed an intermediate polymorphism for all analysed phenotypic groups for Barbari, Sirohi and Black Bengal goats. Effective allele number criterion also revealed equally diverse nature of these genes. This preliminary study provides insight into the genetic diversity of Barbari, Sirohi and Black Bengal goats at GDF9 and BMP4 gene locus, which can be utilized for further study polymorphism and association of these polymorphic variants with traits of economic traits in future.

Key words: BMP4, GDF9, Goat, PCR, Polymorphism, SSCP.

Similar Articles

Systematic review and meta-analysis of the papillomavirus prevalence in breast cancer fresh tissues.
Gomes de Oliveira G, Gonçalves AK, Eleutério J, Pinheiro LGP
Breast disease. 2022; 41(1): 123-132

Clinical Utility of Methylation-Specific Multiplex Ligation-Dependent Probe Amplification for the Diagnosis of Prader-Willi Syndrome and Angelman Syndrome.
Kim B, Park Y, Cho SI, Kim MJ, Chae JH, Kim JY, Seong MW, Park SS
Annals of laboratory medicine. 2022; 42(1): 79-88

Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115(DE3) for Nontransgenic RNAi-Based Insect Pest Management.
Taracena ML, Garcia Caffaro I, Paiva-Silva GO, Oliveira PL, Rendon PA, Dotson EM, Pennington PM
Methods in molecular biology (Clifton, N.J.). 2022; 2360(): 279-294

Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation.
Kaplanoglu E, Kolotilin I, Menassa R, Donly C
Methods in molecular biology (Clifton, N.J.). 2022; 2360(): 235-252

Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR.
Beissner M, Phillips RO, Bretzel G
Methods in molecular biology (Clifton, N.J.). 2022; 2387(): 81-86

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.

ScopeMed Web Sites