Introduction: Cayratia trifolia is a plant that has the potential as a natural antioxidant. The use of natural antioxidant need to be known its toxicity and phenol content. As the limited knowledge of the antioxidant properties and phenolic content of many plant species used as traditional plant medicine. Phenolic molecules and flavonoids are important antioxidant components which are responsible for deactivating free radicals based on their ability to donate hydrogen atoms to free radicals.
Objective: this study aims to assess relationship between the antioxidant activity, toxicity and phenol content of ethanol extract, n-hexane fraction, chloroform fraction and leaf ethanol fraction and Cayratia trifolia stems.
Methods: The antioxidant activity test used the DPPH (2,2-diphenyl-1-pikril-hidrazil) method the total phenol content used the Folin-Ciocalteu method and the toxicity test used the method Brine Shrimp Lethality Test.
Results: The results of this study indicate that the highest % yield is at leaf ethanol fraction 24.52%. The phytochemical screening showed that the extract and ethanol fraction leaves and stems contain alkaloid compounds, flavonoids, polyphenols and steroids. N-hexane fraction and Chloroform leaves and stems contain polyphenol and steroid compounds. There is antioxidant activity in the ethanol fraction of Cayratia trifolia rods has an IC50 value of 58.4 ppm and the highest total phenol content which is 56.2 μg / mL. The toxicity test showed that the chloroform and ethanol fraction was stem had the highest toxicity activity with an LC50 value of 92.4 ppm.
Conclusion: This indicates that the ethanol fraction is active as an antioxidant and the chloroform fraction of the stem is active as an anti cancer.
Key words: Antioxidant, Cayratia trifolia, phenol, toxicity