Abstract

Spinal Tuberculosis is an infection of the spine caused by Mycobacterium Tuberculosis. It is an extrapulmonary complication of Mycobacterium Tuberculosis infection. The purpose of this study was to determine whether there is a NOS 2A gene polymorphism so that patients are susceptible to tuberculosis spondylitis. This study is an analytical cross-sectional study on 36 Spinal tuberculosis patients at the Wahidin Sudirohusodo Hospital in Makassar who had tested positive TB geneXpert and took 5 ml of blood samples in EDTA tubes. Then DNA extraction and genotyping examination with PCR techniques were carried out.
There were 39 subjects in this study 39 TB spondylitis patients (84.8%) and 7 controls (15.2%). Most of them were women (58.7%), aged between 20-29 years (41.3%). Based on PCR results, only 2 subjects experienced a gene mutation (4.3%) in the A-277G single nucleotide. The NOS2A gene polymorphism is a cause of susceptibility to spinal tuberculosis.

Key words: Spinal Tuberculosis, Polymorphism, Inducible Nitric Oxide Synthase, NOS 2A