Infectious bronchitis virus isolate (IND/AHL/16/01) was collected from an outbreak in coloured layer pure line (Dehlam red) and molecularly characterized as nephropathogenic strain by S1 genotyping and phylogenetic analysis. The isolate was experimentally inoculated into chicken by intranasal and intravenous routes @ 104.7 EID50 and after 21 days, birds were euthanized and lungs, kidneys were collected in 2.5% glutaraldehyde for studying the ultrastructural pathology by TEM (Transmission Electron Microscopy). Kidney sections revealed loss of tubular topography, dilation of intertubular junctions, loss of tubular epithelium, swollen nucleus with condensed nucleolus, vacuolation of cytoplasm, electron dense material in cytoplasm and swollen mitochondria and Lung sections showed detached pneumocytes, extensive vesiculation of alveolar epithelial cells. Based on the findings, the virus isolate is known to be nephrotropic, nephropathogenic, inducing a variety of ultrastructural changes in kidneys and lungs.
IBV, Nephropathogenic, Chicken, TEM.
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