Effective centrifugal force and resident time for the primary clarification of the hepatopancreatic tissues of Brown shrimp (Metapenaeus monoceros) homogenate was performed on the optimum recovery of alkaline phosphatase. Relative Centrifugal Force (RCF) of 1681.1×g for 5 min at 4oC successfully clarified 90.28±1.55% of the insolubles. The forces below this level even upto 20 min removed only up to 56.57±0.66% of the total solids and 7.90±0.19% of the total lipids from the homogenate. Force and time above RCF of 1681.1×g for 5 min, specific activity was increased by 4.18±0.38 folds due to the loss of total protein, but upto 60.48±2.61% of alkaline phosphatase activity was lost. Hence, centrifugal force of 1681.1×g for 5 min at 4oC is an effective method of clarification of tissue homogenenate.
Key words: Alkaline phosphatase, Shrimps, insoluble, centrifugation, clarification
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