Nanoencapsulated Java tea-based beverage was previously reported capable of demonstrating the highest protective activity for Langerhans and beta-cell. The mechanism of anti-hyperglycemic activity ,however, has remained unclear. This research aimed to study the mechanism of the beverage as an anti-hyperglycemic encapsulated functional drink through its role in malondialdehyde formation in streptozotocin induced diabetic Sprague Dawley rats. The rats were divided into 4groups: (A) normal rats as negative control, (B) untreated diabetic rats as positive control, (C) diabetic rats treated with microencapsulated drink (3.64 mL/200 g), and (D) diabetic rats treated with nanoencapsulated drink (3.64 mL/200 g). The intervention was conducted for 44 days. Malondialdehyde level, body weight, and blood glucose level were measured periodically every 2 weeks and 1 week respectively for 6 weeks. Malondialdehyde level analysis in vitro was carried out as a comparison. Compared to MDA level on positive control group, encapsulated Java tea-based beverages intervention could attenuate the formation of malondialdehyde in diabetic rats at 36.63% - 51.95%. In addition, micro- and nanoencapsulated drink could suppress the fluctuation of blood glucose level and body weight. In vitro assessment showed that micro and nano-encapsulated suppressed the formation of MDA at 5.25% and72.16%, respectively. The anti-hyperglycemic activity of micro- and nanoencapsulated drink are shown by their ability to alleviate the MDA formation both in vivo and in vitro assessment.
Key words: Diabetes mellitus, nanoencapsulation, microencapsulation, lipid peroxidation, stress oxidative
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