Chaetomium madrascence AUMC 9376 isolated from the soil of Cairo governorate is the most promising isolate that produce biosurfactant using apple pomace as agro industrial substrates. The highest biosurfactant yield was obtained at 7 days incubation, 30oC and pH 8. Moreover, it was revealed that corn steep liquor and Tween 20 are the most suitable sources for optimal biosuractant production. The extracted biosurfactant was fractionated using HPLC resulting in 5 fractions. The purified biosurfactant has a critical micelle concentration of 120 mg/ml. The stability studies revealed that biosurfactanthas a wide range of temperature, pH, and salinity concentration. Additionally, these purified biosurfactant has a strong antimicrobial activity against E. coli, Agrobacterium tumefaciens, Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus flavus and Candida albicans. Moreover, they possess a good antioxidant activity by DPPH scavenging activity at 1.5 mg/ml.
Key words: Chaetomium madrascence, biosurfactant, characterization, antimicrobial activity, antioxidant activity.
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