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Review Article

SRP. 2021; 12(1): 931-934


Molecular Detection Of Klebsiella Pneumoniae Isolated From Different Clinical Source

Boraq Abbas Abd Alwahed, Noor Saad latteef, Wathiq Abbas Hatite Al-Daraghi.


Abstract

The World Health Organization has listed Klebsiella pneumoniae as one of the global priority pathogens in critical need of next-generation antibiotics. Compared to other Gram-negative pathogens, K. pneumoniae accumulates a greater diversity of antimicrobial-resistant genes at a higher frequency. This study included collecting 60 samples from different sources (wounds, Burns, sputum, urine), and the samples were diagnosed by traditional methods, culturing samples on different media, then conducting biochemical tests, and initial tests showed that 50% of the samples were Klebsiella pneumoniae bacteria, and the rest For species (Escherichia coli 30%, Streptococcus pneumoniae 10%, Pseudomonas aeruginosa 5%, Staphylococcu aureus 5%), then Klebsiella pneumoniae was diagnosed by polymerase chain reaction (PCR) using the diagnostic gene (infB). The results showed that only 15 samples out of 30 had this gene. It was diagnosed as Klebsiella pneumoniae, after that CTX resistance gene was examined, and the results showed the presence of this gene in 67% isolates and its absence in 33%. The results showed the presence of this gene in bacteria isolated from urinary tract infection, and with a small percentage of samples isolated from

Key words: Multidrug Resistance; Klebsiella pneumoniae; and PCR






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