Abstract
In this study, we examined the berberine-chitosan nanoparticles (BCNP) action as a natural polymer used to enhance liver and kidney damage induced by phenylhydrazine in male rats. Healthy 20 male Sprague-Dawley rats weighed 150 - 200 g were used in this study and the animals were randomly divided into four groups, G1: (CON), G2: chitosan (CHS), G3: berberine (BER), and G4: berberine-chitosan NPs (BCNPs). Serum ALT, AST, TP, and TB for assessment of liver damage, and creatinine (CRI) and urea (UR) were measured to investigate kidney damage. The administration of experimental rats with BCNPs done by the current study BCNPs with the dose of 0.5 mg/Kg B.W. will be given protection from hepatotoxic and kidney damage action of phenylhydrazine. Liver sections revealed moderate improvement of the hepatic artery, portal vein, and bile duct and reorganization of hepatic cords. Kidney sections illustrated also moderate improvement of tubular necrosis. In conclusion, the usage of BCNPs as a natural polymer with moderated modifications has greatly improved the curative effect of pure BER and CHS to protect the liver and kidneys against toxicity induced by phenylhydrazine that may be by a reduction in lipid peroxidation and enhancement of the main markers of liver and kidney.
Key words: Liver and kidney damage, Berberine, Chitosan, drug delivery,
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