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Original Article



Antimicrobial and Phytochemical screening of selected wild mushrooms naturally found in Garhwal Himalayan region, Uttarakhand, India

Gaurav Kothiyal; Keerti Singh.




Abstract

ABSTRACT
Natural products contains several ingredients that can treat number of ailments. Due to the increase in antibiotic-resistant microorganisms, natural resources are being looked at as an alternative source to combat harmful microbes. This can reduce the effects on harmful microbes by obtaining antibacterial compounds derived from natural resources. In this study hexane, chloroform, methanol, 70% ethanol, and hot water extracts of Cantharellus cibarius, Phellinus Pectinatus, Laccaria laccata, Trametes versicolor, and Gloeophyllum sepiarium were tested for antibacterial activity against nine bacterial strains namely Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aerouginosa, Acinetobacter baumannii, Pseudomonas fluorescens, Enterobacter aerogenes, Proteus mirabilis by disk diffusion method, The microorganisms susceptibility and resistance pattern were tested and measure through the zone of inhibition, which was calculated in millimeter-scale. The Phytochemical analysis was carried out, which represents the presence of active compounds in wild mushroom extracts. The present study reveals important secondary metabolites compounds including alkaloids, flavonoids, carbohydrates, glycosides, etc. were present in wild mushroom extracts. Out of 5 extracts, Methanol and ethanol extract have been shown a great potential as antimicrobial secondary metabolites compared to other extracts. The present results and findings suggested that the wild mushrooms under investigation could be used as a natural antimicrobial source for the inhibition of many bacterial infections.

Key words: Keywords: Antibacterial; Ethonal; Flavonoids; Microorganism; Phytochemical; Wild mushrooms.






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