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Original Research

Preparation of Herbal Formulation of Stevia and Piper Longum and its Antiinflammatory and Cytotoxic Effect

M. Vignesh,S. Rajeshkumar,Anitha Roy,*T.Lakshmi.


AIM: The aim of the study was to prepare the herbal formulation of stevia and the piper longum nanoparticle and to
evaluate its anti-inflammatory and cytotoxic effects.
Materials and Methods: For the evaluation of antiinflammatory activity we used albumin denaturation assay and for the
cytotoxic activity we used a brine shrimp lethality assay and evaluated the result according to the test results and the
graph was made with the results
Result : The nanoparticle of stevia and piper longum showed a marked anti inflammatory and cytotoxic activity with
increased in their concentrations
Conclusion: Stevia and piper longum nanoparticle markedly increase the anti inflammatory and cytotoxic activity compared
to the standard and should be employed as a formulations

Key words: Anti inflammatory, Brine shrimp lethality assay, Cytotoxic activity,Piperlongum Stevia, .

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