AIM: The aim of the study was to prepare the herbal formulation of stevia and the piper longum nanoparticle and to
evaluate its anti-inflammatory and cytotoxic effects.
Materials and Methods: For the evaluation of antiinflammatory activity we used albumin denaturation assay and for the
cytotoxic activity we used a brine shrimp lethality assay and evaluated the result according to the test results and the
graph was made with the results
Result : The nanoparticle of stevia and piper longum showed a marked anti inflammatory and cytotoxic activity with
increased in their concentrations
Conclusion: Stevia and piper longum nanoparticle markedly increase the anti inflammatory and cytotoxic activity compared
to the standard and should be employed as a formulations
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