The experiment was conducted to develop a suitable protocol for high frequency plant regeneration of wild eggplant (Solanum sisymbriifolium) in order to produce a large number of rootstocks for tomato grafting for the management of wilt disease. To obtain in vitro seedlings of S. sisymbriifolium, seeds were treated with various concentrations of GA3 (Gibberellic acid) prior to place them in germination media (˝ strength MS) and 750 mg/L GA3 was found as a suitable concentration resulting the highest (76.67%) germination rate. Various factors namely combination of plant growth regulators, explant types and explant age were investigated for development of an efficient plant regeneration system of S. sisymbriifolium. Cotyledon and hypocotyl explants of S. sisymbriifolium were cultured on MS medium supplemented with various concentrations of BA (6-Benzylaminopurine), NAA (Î±-Naphthalene acetic acid) and 2,4-D (2,4-Dichlorophenoxy acetic acid), to determine suitable medium for callus and shoot initiation. Fourteen days old cotyledon explants were found more responsive than that of hypocotyl, both in callus and shoot induction. The highest callus initiation (100%) and shoot regeneration (73.33%) were observed in MS media supplemented with 0.5 mg/L NAA + 1.0 mg/L BA and 0.2 mg/L NAA + 3.0 mg/L BA, respectively. MS medium supplemented with 0.1 mg/L NAA showed the highest frequency (86.67%) of rooting. The regenerated plantlets were acclimatized in pot soil and eventually used as rootstock for tomato (Solanum lycopersicum cv. BARI hybrid 4) grafting. The grafted plants showed no wilt disease in field condition until maturity.
Wild eggplant, rootstock, tomato, wilt disease, grafting, organogenesis
Exploring the Alzheimer's disease neuroepigenome: recent advances and future trends.
Zhang H, Elefant F
Neural regeneration research. 2022; 17(2): 325-327
Growth differentiation factor 5: a neurotrophic factor with neuroprotective potential in Parkinson's disease.
Goulding SR, Anantha J, Collins LM, Sullivan AM, O'Keeffe GW
Neural regeneration research. 2022; 17(1): 38-44
Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling.
Bertucci T, Kakarla S, Kim D, Dai G
Methods in molecular biology (Clifton, N.J.). 2022; 2375(): 1-12
Presenilin mutations and their impact on neuronal differentiation in Alzheimer's disease.
Hernandez-Sapiens MA, Reza-ZaldĂvar EE, MĂˇrquez-Aguirre AL, GĂłmez-Pinedo U, Matias-Guiu J, Cevallos RR, Mateos-DĂaz JC, SĂˇnchez-GonzĂˇlez VJ, Canales-Aguirre AA
Neural regeneration research. 2022; 17(1): 31-37
SYNGR4 and PLEKHB1 deregulation in motor neurons of amyotrophic lateral sclerosis models: potential contributions to pathobiology.
Marques RF, Duncan KE
Neural regeneration research. 2022; 17(2): 266-270