Reproductive disorders in farm animals represent a great importance due to economic losses for breeder and country economy. The main cause of reproductive failure in cattle is infectious abortion. Brucella, Leptospira, Mycoplasma and Listeria are important zoonosis and leading to sever economic losses in animals around the world, especially in Mediterranean countries and Egypt. Since early detection is crucial in control and treatment, molecular techniques proved to be a more rapid, sensitive and specific diagnostic tool. The present study is aimed to detect these four important pathogens using multiplex- PCR. The multiplex PCR has been standardized by using 4-pairs of primers to amplify 31kDa gene encoding protein in Brucella spp., lig gene in pathogenic Leptospira, hlyA gene in Listeria monocytogenes and 16S rDNA in Mycoplasma spp. A total of 161 different clinical samples were used, an expected band at 223bp, 468p, 370 bp and 270bp were obtained from Brucella spp., pathogenic Leptospira, Listeria monocytogenes and Mycoplasma bovis, respectively. In conclusion, the results of this conventional m-PCR assay revealed that it can be a valuable tool for simultaneous, rapid, cost saving and reliable detection of these microbial agents causing bovine abortion.
Key words: Brucella, Leptospira, Mycoplasma, Listeria, Multiplex-PCR
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