In the present work we have designed short chain α-helical linear and cyclic peptide from cecropin B having same charge, hydrophobicity and helicity. The designed compounds were synthesized by using solution phase method. Elucidation of structure of newly synthesized peptides was done by 1H-NMR, 13C-NMR, FT-IR, FABMS and elemental analysis. Further, the MTT assay was performed for cytotoxicity of synthesized compounds against Daltons lymphoma ascites (DLA), Ehrlichs ascites carcinoma (EAC) and MCF-7 cell lines using 5-FU as reference compound. Biological evaluation showed that short chain cyclicpeptides were more potent than linear peptides against EAC and DLA cell lines.
Key words: Cecropin B, Cationic Peptides, Cytotoxic activity, DLA Cell lines