SRP. 2020; 11(10): 634-634
The Identification of Pork Contamination on Beef by Polymerase Chain Reaction (PCR)
Mustofa Helmi Effendi, Shelma Warda Afdilah, Dhandy Koesoemo Wardhana, Fredy Kurniawan, Rurini Retnowati.
This study aim was to determine the ability of the Polymerase Chain Reaction (PCR) method for identification of pork contamination. The research sample consisted of beef as a negative control, pork which was diluted and mixed with beef as research material to be examined for pork contamination. The validation test method in this study was by testing the sensitivity in serials of dilution of pork (1:101: 1:103; 1: 105; 1: 107). PCR amplification result showed that this method was able to detect pig DNA contamination in 1: 105 pork dilution. It can be concluded that PCR has the ability to detect pork contamination on beef quite sensitive and accurate.
Key words: Pork contamination, PCR, sensitivity, pig DNA