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Extraction of protein concentrate from red bean (Phaseolus vulgaris L.): antioxidant activity and inhibition of lipid peroxidation

Lucrecia Pi˝uel, Edgar Vilcacundo, Patricia Boeri, Daniel Alejandro Barrio, Dayana Morales, Adelita Pinto, Roberto Moran, Ivan Samaniego, Wilman Carrillo.

Abstract
The antioxidant activity of Red Bean Protein Concentrate (RBPC), and RBPC hydrolysates was evaluated. The RBPC protein content was in the range of 57.38% to 72.68% of the total sample content. RBPC protein profile showed a range of 15 kDa to 100 kDa. Phaseolin protein was the main fraction with a molecular weight of 45 kDa and 50 kDa. Phaseolin protein was found in all RBPC samples at the different pHs assayed. In the gastric digestion phase, bands from 60 kDa to 100 kDa were totally hydrolyzed with pepsin. Phaseolin protein (45 kDa and 50 kDa) presented resistance to gastric hydrolysis. All RBPCs and gastrointestinal digest presented antioxidant activity using FRAP, ABTS and ORAC and TBARS using the in vitro and in vivo methods. RBPC at pH 7.0 presented a value of 95.80 ÁmoL TE/ g of RBPC (FRAP); 257.12 ÁmoL TE/ g of RBPC (ABTS) and 1960 ÁmoL TE/ g of RBPC (ORAC). Duodenal digest of RBPC presented high antioxidant activity with 225.77 ÁmoL TE/ g of digest (FRAP); 345.21 ÁmoL TE/ g of digest (ABTS) and 3256 ÁmoL TE/ g of digest (ORAC). Gastric and duodenal digest of RBPC were used to inhibit lipid peroxidation using the in vitro method presenting a value of 87.95% and 93.0% respectively. When the in vivo method in zebrafish larvae was used, values were 79.03% and 86.76% respectively. RBPCs showed no ROS inhibition. However, RBPCs with gastric and gastrointestinal digests, presented ROS inhibition, 75.30% for gastric digests and 66.40% for gastrointestinal digests.

Key words: common bean; Phaseolus vulgaris; protein concentrate; antioxidant activity; zebrafish larvae


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