Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Research



Colloidal Dye Immunobinding Assay for Detection of Trypanosoma evansi Antibodies in Animals

S.SIVAJOTHI, V.C.RAYULU, P.MALAKONDAIAH, D.SREENIVASULU.

Cited by (11)

Abstract
Colloidal dye immunobinding assay (CDIA) was developed for detection of circulatory antibodies of Trypanosoma evansi in the sera of domestic animals. Specific antibodies in the serum were captured by T. evansi antigen spotted on to nitro cellulose membrane mounted on a flow through device. The bound antibodies were visualised by the addition of protein A colloidal gold conjugate which imparts pink colour to the membrane. The optimum concentrations of antigen, test serum and colloidal dye protein A conjugate in the assay were standardized. The efficacy of CDIA was determined by comparing with the results of indirect ELISA. CDIA could detect T. evansi antibodies in 261 ﴾cattle (n=92), buffaloes (n=132) and sheep (n=37) ﴿ serum samples out of 290 ﴾cattle (n=100), buffaloes (n=140) and sheep (n=50) ﴿ indirect ELISA positive samples. All the 22 samples found positive for T. evansi infection by Wet blood film (WBF) examination were also found to be positive by CDIA. The diagnostic sensitivity and diagnostic specificity of CDIA were recorded as 90.00 and 100 per cent, respectively. The CDIA developed in the present study for detection of T. evansi infection in domestic animals is simple to perform, rapid, could potentially applied in all host species, economical and suitable for a wide variety of field applications without any sophisticated instrumentation.

Key words: Trypanosoma evansi; colloidal dye; immunobinding assay; antibodies.



Similar Articles

Simultaneous adsorption for cationic and anionic dyes using chitosan/electrospun sodium alginate nanofiber composite sponges.
Zhao X, Wang X, Lou T
Carbohydrate polymers. 2022; 276(): 118728

Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded Under Neurosphere Culture.
Koopaei NN, Schmittgen TD, Reynolds BA, Azari H
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 87-94

Isolation and Culture of Adult Hippocampal Precursor Cells as Free-Floating Neurospheres.
Rust R, Walker TL
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 33-44

A sensitive electrochemiluminescence biosensor for assay of cancer biomarker (MMP-2) based on NGQDs-Ru@SiO2 luminophore.
Fan X, Wang S, Liu H, Li Z, Sun Q, Wang Y, Fan X
Talanta. 2022; 236(): 122830

Culturing Mouse Fetal Neural Precursor Cells in a Free-Floating Serum-Free Condition.
Azari H
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 1-10


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites