Symbiotic association between marine sponge and microorganism was a promising chance in the discovery of lead compound of anticancer. This association was probably concluded that symbiotic microorganism would contain the same secondary metabolites with the host. In this continuation research, we had isolated symbiotic bacteria from a marine sponge and tested for cytotoxic activity. Twenty-six isolates of bacteria derived marine sponge Haliclona fascigera was isolated from Setan Island, West Sumatra, Indonesia. Screening of cytotoxic activity by BSLT method and MTT assay was conducted toward twenty-one ethyl acetate extracts of symbiotic bacteria with weight >50 mg. One bacteria extract with code H2N was very toxic according to BSLT test, while eighteen isolates were toxic with LC50 ranging from 31.17 to 283.38 ppm. All of the bacteria extracts did not show a good percentage of viability (>50%) against Hela, WiDr, T47D, dan Vero cell line in MTT assay. However, bacteria extract with code H2N have shown potential cytotoxic compared other extracts. Due to phytochemical study this extract probably contained terpenoid group. Based on biochemical examination this bacteria, H2N, was identified as Bacillus sp.3.
Key words: Bacillus sp., cytotoxic activity, Haliclona fascigera, marine-derived bacteria, marine sponge
|
