Andrographis paniculata is a well-known and highly used potential herbal plant for worldwide medicinal use. Plant studies reported that the leaf extract of A. paniculata, which has high antioxidant capacity, has antifungal, antimicrobial, antiprotozoal, antidiabetic, liver enzyme modulation, insecticidal, and toxicity properties. Nevertheless, no report regarding its antimelanogenic activity is available. Hence, this study was carried out to investigate A. paniculata leaf extract potential of the antimelanogenic property. The data shown from this study proved that the A. paniculata leaf extract is responsible for melanin content reduction and decreases the protein expression of tyrosinase (TYR) and microphthalmia-associated transcription factor in B16F1 melanoma cells. This study found that the A. paniculata leaf extract acted powerfully in the synthesis of tyrosinase-related protein-2 inhibition. TYR is a well-known essential enzyme in melanogenesis activity. These results represent that the A. paniculata leaf extract strongly decreases the expression level of the enzyme important for melanin synthesis. Therefore, the A. paniculata extract has a high capability of becoming a lightening agent and hyperpigmentation treatment in the pharmaceutical and cosmetic market.
Key words: Andrographis paniculata leaf extract, melanogenesis, B16F1, tyrosinase, MITF, hyperpigmentation
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