Sesbania sesban Linn belongs to the Fabaceae family, which is found in tropical and subtropical parts of the world. Other members of this family include Sesbania aculeata, Sesbania drummondii, Sesbania grandiflora, Sesbania rostrata, and Sesbania speciosa. The goal of this research is to conduct a phytochemical analysis as well as an in-vivo anti-inflammatory, in-vitro antioxidant and anticancer investigation of phytoactive extracts. All sections of the plant were examined for organoleptic characteristics, additional features, and macroscopical details. The foreign organic matter, moisture content, total ash value, water-soluble ash value, acid-insoluble ash value, water-soluble extractive value, and alcohol soluble extractive value of powdered Sesbania sesban leaves were all evaluated. For improved separation of the components, n-butanol extracts of Sesbania sesban leaves were treated to TLC using a suitable solvent system. The presence of secondary metabolites such as flavonoids, Glycosides, tannins, and phenolic substances is confirmed by TLC analysis of extracts. Furthermore, the TLC of flavonoid and glycosides was highly resolved, indicating that they were present in greater quantities. In-vivo anti-inflammatory, In-vitro antioxidant and anticancer studies (Cell line-A549) on phytoactive extracts (SS3, SS4) were conducted and shown to have excellent efficacy.
Key words: Extraction, characterization, Sesbania sesban, in-vivo anti-inflammatory activity, in-vitro antioxidant study, anticancer study
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