Oxidative stress is a pathological state that is responsible for the growth of chronic disorders like arteriosclerosis, cancer, diabetes mellitus, liver injury, inflammation, skin damages, coronary heart diseases, and arthritis. Antioxidants are substances that when found in food or herbs, that stop and stabilize the oxidative damage triggered by free radicals by providing electrons from antioxidants to these damaged cells. Ayurveda is said to be holistic as it objectives to integrate and balance body, mind, and spirit to stop illness and promote wellness, longevity, vitality, and happiness, hence in the current research we are planning to prepare and assess the efficacy of the total antioxidant potential and radical scavenging activity of a polyherbal formulation. The total antioxidant power and free radical scavenging activity of polyherbal formulation was assessed by Total Antioxidant Capacity by Phosphomolybdate Assay and DPPH Scavenging Activity. In Phosphomolybdate Assay the polyherbal formulation PHF2 shows the increasing total antioxidant capacity equivalence to Ascorbic acid per gram (µg AAE/g) with increasing concentration as compared to polyherbal formulation PHF1 and PHF3. While in DPPH free radical Scavenging assay, the polyherbal formulation (PHF2) shows highest free radical scavenging activity with IC50 = 43.57 µg/ml when evaluated with standard Ascorbic acid 48.25 µg/ml, PHF3 62.75 µg/ml and PHF1 88.15 µg/ml. Hence, the evolved polyherbal formulation might substantiate to be a safe alternative treatment for the management of chronic metabolic disorders like diabetes, cancer, liver injury, inflammation, skin damages, coronary heart diseases, and arthritis through reducing oxidative stress.
Key words: Oxidative Stress, Chronic Disorder, Polyherbal Formulation, Ascorbic Acid, IC50
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