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In vitro and in vivo effect of Eurycoma longifolia root extract on UDP-glucuronosyltransferase activity

Purwantiningsih Purwantiningsih, Abas Hj Hussin, Sabariah Ismail, Kit Lam Chan.


The aim of this research was to evaluate the effects of E. longifolia extract (ELE) on enzymes activity in the phase II drug metabolism on Sprague-Dawley (SD) rats especially on UGT activity, in-vivo and in-vitro. The UGT assay was performed based on method that Bock & Kohle described (2004). In the in-vivo study, the male SD rats were treated with ELE at doses ranging from 5 to 1000 mg/kg b.w. and 5, 25 and 50 mg/kg b.w for acute and sub-acute study, respectively. The ELE concentrations of 0.01 to 1000 g/mL were used for in-vitro study. The pNP that consumed in the glucuronidation process reflected the UGT activity and was calculated using standard curve of pNP. UGT enzyme activity was inhibited significantly (P < 0.05) by ELE extract in the male rat for both acute and sub-acute experiments. ELE extract decreased the enzyme activity significantly (P < 0.05), at all concentrations tested in the in-vitro experiment when compared to the negative control group. ELE had a lower activity (the IC50 of 0.74 g/mL) as compared to Na diclofenac (positive control) with an IC50 of 0.17 g/mL. E. longifolia decreased the UGT enzyme activity significantly (P

Key words: Eurycoma longifolia, phase II drug metabolism, UGT enzyme activity

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