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Cytotoxic and Genotoxic effects of Orthodontic Adhesives on Human lymphocyte - An In-vitro Study

Ravi M S, Vijay R, Suchetha Kumari N, Chirag Panchasara.


Aim of this study was to evaluate the in vitro genotoxicity and cytotoxicity of two orthodontic adhesives and to determine the type of cell death they induce on human lymphocytes. The materials tested were 1.Light cure orthodontic adhesive with conventional primer (Transbond XT3M) and 2. Self cure orthodontic adhesive (Unite, 3M). Cured sterile individual masses were immersed in DMEM and left at 370C for 24 h. Then a volume of 200 μL of the extract medium was mixed with human peripheral blood lymphocyte tested for comet assay by Single cell DNA Damage assay and Apoptosis by DNA diffusion agar assay. Evaluation of Cytotoxicity was carried out by Hemolysis assay method. Hemolytic activity of Self cure orthodontic adhesive (52.9± 1.82) was significantly more than that of Light Cure orthodontic adhesive (48.9±1.23). The results showed all parameters studied by comet assay were significant (P>0.05).In Case of Apoptosis, Light cure orthodontic adhesive (155.11±6.03) and self cure orthodontic adhesives (154.77±13.17) showed slightly increased diffusion of DNA compared to normal lymphocyte (111.22±8.78).However the level of DNA diffusion was not significantly different between the two adhesives. Light Cure orthodontic and self cure orthodontic adhesives were cytotoxic and induced apoptosis. The self cure orthodontic adhesive was found to be significantly more toxic than that of Light Cure orthodontic adhesive. Both the adhesives had no significant effect on the percentage of DNA tail and Tail length of the human lymphocyte.

Key words: Orthodontic Adhesives, DNA Damage, Apoptosis, Lymphocyte.

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