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Heme Oxygenase-1 Expresion in Gastric Mucosa and Liver after Burns: Preliminary Immunohistochemical Study

Ganka Bekyarova, Maria Tzaneva, Minka Hristova.

Abstract
Objective: Generation of reactive oxygen metabolites (ROM) and oxidative stress are crucial in pathogenesis of thermal skin injury. Heat shock proteins such as HSP32 known as heme oxygenase-1 (HO-1) possesses antioxidant, anti-inflammatory, and vasodilative properties and play an important role in the protecting of tissues from several stresses. In this immunohistochemical study, we have assessed the constitutive expression of HO-1 in normal gastric mucosa and liver and tested the hypothesis that its expression in these tissues is upregulated in thermal skin injury characterized by increased production of ROMs and other pro-inflammatory mediators. We used malondialdehyde (MDA) as a marker of oxidative stress and tumor necrosis factor (TNF) α as a marker of inflammatory response in burn rat model.
Methods: HO-1 expression in formalin-fixed sections was assessed by peroxidase anti-peroxidase immunohistochemistry using a polyclonal rabbit anti-HO-1 as primary antibody. TNF-α in plasma was quantified using enzyme-linked immunosorbent assay (ELISA) kit. Spectrometric method was used for plasma and tissue MDA assay.
Results:Immunohistochemical staining revealed HO-1 expression in all groups. Staining scores for HO-1 in endothelial cells in both gastric mucosa and liver were increased with 32% (p

Key words: Heme oxygenase-1; gastric mucosa; liver; burns; inflammatory response; oxidative stress


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