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Coelomic fluid of Lumbricus rubellus enhances anti-prolioniferative effect of 5-fluorouracil by modulating focal adhesion kinase express and IL-1β of colorectal cancer in mice

Sofy Permana,Reny Purnama Hadi,Eviana Norahmawati,Agustina Tri Endharti.

Cited by 4 Articles

The various cancer-promoting mechanisms that associated with Focal adhesion kinase (FAK) can be implicated in the progression of colorectal cancer. A combination treatment of 5-Fluouracil (5-FU) and earthworm could increase the sensitivity of cancer cells by inhibiting proliferation mechanisms. This study was aimed to investigated the anti-proliferative effect of chemoterapy drugs combined coelomic fluid derived from Lumbricus rubellus. Colorectal cancer was actuated in mice by injected HT-29 cells in caecom of mice, followed by administration of drinking water containing 5% dextran sodium sulfate (DSS). This study consists of five treatment groups: group 1 was untreated (control), group 2-5 were treated by 5-FU. Group 3-5 were treated by combination of 5-FU (50 μg/g BW) and coelomic fluid (50 μg/g BW, 100 μg/g BW and 200 μg/g BW), respectively. The administrated of coelomic fluid were started from the 4th weeks, were continued until 8 weeks. At the end of 8 weeks of the experiment, mice were sacrificed, colon tissue was removed, and then subjected to flow cytometry and immunofluorescent examination. These studies showed that combination treatment of colorectal cancer cells with coelomic fluid from Lumbricus rubellus able to inhibit cell proliferation. The combination of 5-FU and coelomic fluid significantly inhibit proliferation, IL-1ß and FAK compare with 5-FU only group (P

Key words: Coelomic fluid, Colorectal Cancer, Focal Adhesion Kinase, IL-1ß, Proliferation

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