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Effects of post-thaw incubation time on sperm functional dynamics and mitochondrial activity in frozen-thawed ram semenŞeyma Özer Kaya, Edip Toraman, Mustafa Sönmez, Tutku Can Acısu, İbrahim Halil Güngör, Nida Badıllı, Seyfettin Gür, Recep Hakkı Koca, Nurdan Coşkun, Tuba Bayır. Abstract |  Download PDF | |  Post | Background:
The cryopreservation process can negatively affect the functional integrity of thawed ram spermatozoa. Evaluating post-thaw changes in sperm motility, kinematic characteristics, sperm subpopulations, and mitochondrial activity may improve sperm quality and functional stability assessment.
Aim:
This study aimed to evaluate the effects of post-thaw incubation time on sperm motility, kinematic parameters, sperm subpopulations, and high mitochondrial membrane potential (HMMP) in frozen-thawed ram sperm.
Methods:
This study used 48 frozen semen straws obtained from 6 breeding rams. Sperm samples were evaluated 0, 1, and 2 h after thawing. Motility and kinematic analyses were performed using a computer-aided sperm analysis (CASA) system, while HMMP ratios were determined by flow cytometry using the 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining method. Functional sperm subpopulations were created based on velocity classes and k-means clustering analysis using CASA data.
Results:
The total and progressive motility rates and percentage of spermatozoa with HMMP decreased significantly depending on post-thaw incubation time (P
Key words: CASA kinematics; Frozen ram semen; Mitochondrial membrane potential; Post-thaw incubation; Sperm subpopulation.
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