Abstract

Soybean oil (SO) is used worldwide, whereas mustard oil (MO) consumption is generally limited to South Asia. Nevertheless, the organ-damaging effects of these substances at multiple doses over time have not been well studied. This study evaluated the hematobiochemical and histopathological effects of SO and MO in a mouse model. A total of 84 male Swiss albino mice, aged 5 weeks, were divided into seven groups (n=12 each): a control group (C) given pellets, and six treatment groups (S1-S3 and M1-M3) administered escalating doses of SO or MO at 15, 25, and 35 ml/kg for 60 days. Following euthanasia, blood samples were examined for hematological and biochemical parameters using standard assays. The hearts, livers, and kidneys were collected for measurement and then prepared for histopathology using H&E staining. The hematobiochemical parameters were significantly elevated in all treated groups when compared to the control group (P

Key words: Hematobiochemical, Histopathology, Mustard oil, Soybean oil, Vital organs, Mouse