Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Research



Cytomorphometric Analysis of Oral Premalignant and Malignant Lesions Using Feulgen Stain and Exfoliative Brush Cytology.

Priya Shirish Joshi, Manasi Sandipak Kaijkar.

Cited by (1)

Abstract
Introduction: Oral squamous cell carcinoma (OSCC) is the sixth most common cancer worldwide and accounting for 90% of cancers of oral cavity. Tobacco abuse has been proved to be the major risk factor in the development of OSCC. Despite advances in surgery, radiation and chemotherapy, the five year survival rate for oral cancer has not improved significantly over the past several decades and it remains at about 50 to 55%. Cytobrush sampling is more frequently used nowadays for exfoliative cytology, since it maximizes the number of cells obtained, and facilitates their uniform distribution onto the microscope slide, thus probably improving sensitivity.Our study was therefore carried out to analyze the cytomorphometric features of cells obtained by cytobrush and stained with Feulgen stain from oral premalignant and malignant lesions and to find out whether these features could be used to detect dysplasia and malignancy in their early stages.
Aims: To analyze the cytomorphological features of cells in smears of oral premalignant and malignant lesions obtained from exfoliative brush cytology using Feulgen stain and to assess the efficacy of the same in detecting dysplasia and malignancy.
Methods: Our study comprised of clinically and histopathologically diagnosed sixty cases which were grouped into twenty cases each of tobacco users with lesions (Leukoplakia and Erythroplakia) (Group I); tobacco users without lesions (Group II); Oral squamous cell carcinoma (OSCC) lesions (Group III); and normal mucosa (Group IV). The epithelial cells from the lesion were collected with a cytobrush and smears were stained with Feulgen stain. The cells were measured using software for their nuclear area, nuclear diameter, cellular area, cellular diameter and nuclear to cellular area ratio (N:C).
Results: The exfoliated cells showed similar alterations as those occuring in histopathological sections of premalignant and malignant lesions. The N:C ratio, mean nuclear area and diameter value was highest in Group III and lowest in Group IV. The mean cellular area and diameter was highest in Group IV and lowest in Group III. Tukey-HSD formula for pairwise comparison showed a significant difference in mean values of nuclear and cellular area and diameter and N:C ratio between all the groups except in Group I and Group III.
Conclusion: Our study was able to differentiate dysplastic and malignant cells from normal ones using analysis based on nuclear and cellular parameters. We therefore conclude that cytomorphometric analysis using exfoliative brush cytology can be of great value for monitoring and follow up of suspicious lesions and can provide an excellent additional diagnostic test for detecting early oral malignancy.

Key words: Cytobrush; Cytomorphometry; Exfoliative cytology; Feulgen stain; Oral squamous cell carcinoma (OSCC)



Similar Articles

Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue.
Curtis MA, Vedam-Mai V
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 201-208

Highly sensitive detection of multiple proteins from single cells by MoS-FET biosensors.
Wei J, Zhao Z, Lan K, Wang Z, Qin G, Chen R
Talanta. 2022; 236(): 122839

Identifying Neural Progenitor Cells in the Adult Human Brain.
Park TIH, Waldvogel HJ, Montgomery JM, Mee EW, Bergin PS, Faull RLM, Dragunow M, Curtis MA
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 125-154

Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny.
Azari H
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 111-123

Improving cell transplantation by understanding and manipulating the phagocytic activity of peripheral glia.
Nazareth L, St John J, Ekberg J
Neural regeneration research. 2022; 17(2): 313-314


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites