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SRP. 2021; 12(1): 254-258

Using Of Area Under The Curve Method For The Spectrophotometric Determination Of Betamethasone Sodium Phosphate In Its Pure And Pharmaceutical Forms

Khalaf F. Alsamarrai, Harith Jameel Mahdi Alsamarraie, and Menaa AbdulSalam Al-Abbasi.


A new, fast, accurate, economic, sensitive spectrophotometric method for the determination of Betamethasone (BSP) was developed. The method includes the use of the area under the curve (AUC) of the BMT UV-spectrum in methanol. This method was very suitable to the determination of BSP because it gave good linearity which up to 10-100 µg/ml, good accuracy and precision, through Rec% values which were ranged between 98.40914-101.40864% and RSD% values were ranged between 0.05507-0.97609%, LOD was 0.029701 µg/ml and LOQ was 0.098013 µg/ml. The method successfully applied to the determination of BSP in pure and trading forms.

Key words: Spectrophotometric, Determination, Betamethasone, Area, Curve, pharmaceutical forms

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