This study aims to introduce a novel method that included zwitterion chromatography-hydrophilic interaction chromatography with a UV detection system for analysis and identification of the fisetin in black and green teas. Flavonoids like fisetin have been shown to have multiple beneficial effects on human health that are available in many fruits, beverages, herbs, vegetables, and nutritional supplements herbs are the main source of flavonoids. Hydrophobic and hydrophilic interactions forming a fisetin mechanism to distinguish mixed mode. The validated process has been successfully used for the extraction test in herbs. Fisetin has been separated in the ZIC-HILIC column using acetonitrile and sodium acetate buffer as the mobile phase. The results showed that the HILIC mode was simple and effective, and could be used to identify the fisetin content in samples of black tea leaves and green tea leaves. The calibration curve was produced in commercial ZIC-HILIC column and linear range (0.01-7 μgmL-1), RSD% (1.32), LOD (0.008 μgmL-1), LOQ (0.024 μgmL-1).
Key words: Fisetin, Black tea, Green tea, hydrophilic interaction chromatography, flavonoids