Our study describes a novel, fast, and accurate method for estimating metoprolol (MET) in human blood plasma using LC-MS/MS technique. To prepare the samples, we used MET d7 as an internal standard (ISTD) and isolated MET from plasma using a special LLE technique. Following evaporation and reconstitution, the samples were injected into a specific chromatography column. Using a technique called single reaction monitoring; we were able to detect MET and the ISTD without interference from other blood plasma components. MET produced a unique signal and the ISTD produced a slightly different but related signal. Our analysis showed a reliable relationship between the amount of MET and the signal strength (correlation coefficient ≥ 0.9956). This relationship held true across a range of MET concentrations. Additionally, tests confirmed that MET remained stable in blood plasma under various conditions, including room temperature storage, injector storage, freeze-thaw cycles, and long-term freezing. This newly developed method adheres to validation guidelines set by the USFDA. It can be effectively used to measure MET levels in blood plasma for both routine testing and pharmacokinetic studies.
Key words: Metoprolol, LC-MS/MS, human plasma, method validation, stability studies.
|
