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Review Article

JCR. 2020; 7(1): 41-47

Rapid Detection of Dengue Virus Transovarial Transmition from Nature and Artificial Inoculation

Isna Hikmawati, Hendro Wahjono, Martini Martini, Soeharyo Hadisaputro, Edi Darmana, Kisdjamiatun Retna Mustika Djati, Sugeng Juwono Mardihusodo, Siti Rahmah Umniyati.

Background: Dengue Hemorrhagic Fever (DHF) is still a major disease problem. Eradication of DHF through vector control has not been able to cut transmission from mosquito to mosquito because there is still empirical evidence of transovarial transmission. The purpose of this study is to rapid detection of dengue transovarial virus transmission from nature and artificial inoculation.
Method: Mosquito eggs obtained from ovitraps installation in several cities are hatched and maintained until they become mosquitoes at a week old without blood feeding, then the presence of DENV-1,2,3,4 is detected based on the RT-PCR method. As a positive control, 2-3 days old Aedesaegypti mosquitoes were injected with DENV-1,2,3, and 4, intra-thoracically under a dissecting microscope. The injected mosquito is kept in a cylindrical cage. Furthermore, mosquito control was developed until progeny 1 (F1), after adult dengue virus was detected by PCR realtime
Results: The results showed the proportion of transovarial events from nature was 60%. The results of transovarial transmission by artificial inoculation showed differences in viral load variations in serotype
Conclusion: Transovarial transmission from nature, found various serotypes with DENV-4 serotype as the dominant serotype. Whereas transovarial transmission through artificial inoculation shows transovarial presence in progeny one (F1) with different viral load values for each serotype. Further research can be analyzed for viral load up to progeny with zero squen quantity, to see its role in maintaining the virus during the interepidemic period.

Key words: Transovarial, Dengue, Serotype, Nature, Artificial, Aedes

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