Abstract

Background
Capparis erythrocarpos Isert has been reported to have anti-dyslipidemic effect on normoglycemic animals. However, there are no reports on its effect on the control of blood sugar levels in diabetic animals.
Objective
This study aimed at investigating the in-vivo antidiabetic activity of C. erythrocarpos in streptozotocin (STZ) induced diabetic mice.
Methods
Plants materials were collected in April 2018 and the study was conducted from April to July 2019. Antidiabetic activity of hydroethanolic extract of C. erythrocarpos was determined at different doses of 250, 500 and1000 mg/kgbwt and compared to positive and negative controls. Acute antihyperglycemic activity in normal mice was investigated by Oral Glucose Tolerance Test (OGTT) method. Safety of the extract was evaluated by the use of “Up and Down” oral acute toxicity testing method. Statistical differences between groups were assessed by Student’s t-test and one-way ANOVA with Tukey’s multiple comparisons.
Results
C. erythrocarpos produced significant reduction in fasting blood glucose level compared to the negative control in STZ-induced diabetic mice after 14 days of daily oral doses of the extract at 250 mg/kgbwt (p=0.0267), 500 mg/kgbwt (p=0.0002) and 1000 mg/kgbwt (p=0.0011). OGTT showed that the extract significantly lowered blood glucose levels 2 hours after oral glucose load at all doses provided 100 mg/kgbwt (p=0.0322), 200 mg/kgbwt (p=0.0118) and 500 mg/kgbwt (p=0.0222). There were no statistically significant difference in clinical signs and symptoms of acute oral toxicity observed between control and treatment groups.
Conclusion
Oral administration of hydroethanolic root extract of C. erythrocarpos is non-acutely toxic and exhibited significant hypoglycemic activity in STZ-induced diabetic mice.

Key words: Acute toxicity, Anti-diabetic, Capparis erythrocarpos, Streptozotocin