In Vietnam and in other Asian countries, the above-ground parts of Pogostemon auricularius (L.) Hassk. (Lamiaeae), having a different range of ethnopharmacological traditions, are used as a natural cure with antiseptic, analgesic and anti-inflammatory properties. This study pointed out the anti-inflammatory effects of some fractions and isolated compounds from P. auricularius.
This study aims to examining the anti-inflammatory effects of certain fractions and isolated compounds from P. auricularius by measuring various pro-inflammatory factors production.
The anti-inflammatory effects of methanol extract, n-hexane, dichloromethane, ethyl acetate and aqueous fractions, as well as Pogostemins A-C, and Pogostemon A-C isolated from above-ground parts of P. auricularius were ascertained by measuring the quantity of pro-inflammatory factors such as interleukin (IL)-6, tumor necrosis factor (TNF)-α, nitric oxide (NO) and anti-inflammatory IL-10 produced by lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
The fractions of ethyl acetate and dichloromethane displayed significant activity contrary to the production of nitric oxide (NO) having IC50 values of 25.28 and 28.68 µg/mL, respectively. The new compound, pogostemin C, had the strongest inhibitory effect with an IC50 value of 1.36 µg/mL. Besides, pogostemin C displayed the ability to decrease pro-inflammatory TNF-α and increase anti-inflammatory IL-10 in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
This study reveals that the fractions and pogostemin C extracted from the above-ground parts of P. auricularius can inhibit the production of nitric oxide and TNF-α, whereas activate anti-inflammatory IL-10 production, thereby explaining the ethnopharmacological traditions of this herb.
Key words: Inflammation; IL-6; IL-10; nitric oxide, Pogestemon auricularius; pogostemin C; TNF-α;