Candida albicans is one of the most common fungal pathogens of humans and has become the fourth leading cause of nosocomial infection. This work was carried out to study pathological and some immunological reactions in C. albicans artificial infection. Forty eight mice were divided into three groups (one control and two test groups). Mice of group one injected with 0.2 ml broth containing (106 org/ml) of Candida albicans with 0.2 ml of hydrocortisone at the same time intraperitonially. Second test group were injected intraperitoneally with 0.2 ml broth containing (106 org/ml) of Candida albicans only. Control group were injected by 0.2 ml of sterile normal saline intraperitoneally. Mice were observed and managed during the period of the study extended from January 2009 to June 2009. Mice were sacrificed after different periods (7, 14, 21, 28, and 35 days of infection) for histopathological study. Blood was collected from each mouse. Sera were employed for antibody titration using ELISA test. Candia albicans was recovered from all organs within 1-day post infection. The number of C. albicans in the hearts and livers declined rapidly with time. Histopathological lesions were extensive with multiple foci of hyphal invasion in kidneys, hearts, brains and spleens of infected mice. Antibody titer levels detected by ELISA through the time are demonstrated. High titers were demonstrated in immunocomptent group. The study indicated that antibody response after second challenge with C.albicans was lower in the immunocompromized than in nonimmuno-compromized. Pathological changes were the same in both groups.
Key words: Candida, Pathogenesis of Candida albicans, Immunological study of Candida albicans