Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Article

Isolation, Characterization and Proliferation of Cancer Cells from Breast Cancer Patients

Wahyu Widowati, Yusuf Heriady, Dian Ratih Laksmitawati, Diana Krisanti Jasaputra, Teresa Liliana Wargasetia, Rizal Rizal, Fajar Sukma Perdana, Annisa Amalia, Annisa Arlisyah, Zakiyatul Khoiriyah, Ahmad Faried, Mawar Subangkit.

Introduction: The most cases of cancer death, which are in the first rank among cancers suffered by women is breast cancer. The breast cancer therapy for patients has been done, but still not optimal, so it is necessary to understand the mechanism of therapy in model cell of breast cancer. Aim: This study aim to develop an isolation technique of breast cancer cell from patients as a cancer cell model. Material and Methods: Breast cancer cell isolation is performed by enzymatic methods using the collagen I and hyaluronidase. Then, breast cancer cells were characterized using flow cytometry based on the CD44/CD24 expression where MDA-MB468 and MCF-7 breast cancer cell lines were used as positive controls. Estrogen receptor (ER), progesterone receptor (PR), p53, HER2, and Ki67 expression were assessed using an immunohistochemistry assay. Result and Discussion: The morphology of cancer cells was fibroblast like cells on the day 7th after isolation. Isolated breast cancer cells expressed 95.330.47% of CD44+/CD24+ and human epidermal growth factor receptor 2 (HER2) low expressions. Isolation of breast cancer cells can use In-house enzymatic protocol. Isolated breast cancer showed the same expression as MDA-MB468 (CD44+/CD24+) and HER2- compared to MCF-7 cell lines (CD44-/CD24+). Conclusion: These cells belonged to a basal type of breast carcinoma and expressed CD44+/CD24+, then isolated BCCs can be used as model cancer cells for further research.

Key words: breast cancer, cell lines, CD44 antigen, CD24 antigen, enzymatic assay.

Similar Articles

Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue.
Curtis MA, Vedam-Mai V
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 201-208

Highly sensitive detection of multiple proteins from single cells by MoS-FET biosensors.
Wei J, Zhao Z, Lan K, Wang Z, Qin G, Chen R
Talanta. 2022; 236(): 122839

Identifying Neural Progenitor Cells in the Adult Human Brain.
Park TIH, Waldvogel HJ, Montgomery JM, Mee EW, Bergin PS, Faull RLM, Dragunow M, Curtis MA
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 125-154

Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny.
Azari H
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 111-123

Improving cell transplantation by understanding and manipulating the phagocytic activity of peripheral glia.
Nazareth L, St John J, Ekberg J
Neural regeneration research. 2022; 17(2): 313-314

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.

ScopeMed Web Sites