Abstract

Silver nanoparticles were synthesized from 1mM aqueous silver nitrate through an ecofriendly and cost effective method by
using Tinospora cordifolia stem powder. The bioreduction behavior of stem powder of Tinospora cordifolia is responsible
for this biosynthesis. The stem powder extracts mixed with silver nitrate showed gradual change in the color of the extracts
from yellow to dark brown. The formation of silver nanoparticles was confirmed by UV-Visible spectrophotometer, X-Ray
diffraction (XRD), Fourier transform infrared (FTIR), Energy dispersive spectroscopy (EDAX) and Transmission electron
microscopy (TEM). Silver nanoparticles have been known to have bactericidal and inhibitory effects. Resistance to
antibiotics by pathogenic bacteria has emerged in recent years and is a major health problem. The effects of silver
nanoparticles and different antibiotics on antibiotic resistant bacteria have not been studied.

Key words: Silver nanoparticles, Tinospora cordifolia, antibacterial activity, Energy dispersive spectroscopy (EDAX), XRay diffraction (XRD), Fourier transform infrared (FTIR), Antibiotic resistance bacteria.