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Review Article

IJPRT. 2014; 4(2): 10-17

Formulation, Evaluation and Validation of Newly Formulated Laportea Arishta

Deepa Padmaja*, Manikandan Palanivelu, Babu Ganesan, Seena, Arunraj Natarajan.


Laportea Arishtas are self generated herbal fermentations made with decoctions of Laportea interrupta leaves. The
improvement in the extraction of drug molecule from the herb, improvement in drug delivery into the human body sites and
enhancing therapeutic properties of the herb are the main reason for selecting this herb. Physico chemical analysis was
performed for the crude drug for assuring its authenticity. The formulated arishta were subjected to organoleptic-physicochemical
analysis, qualitative and quantitative analysis, antioxidant studies along with a new analytical method validation.
The quantitative estimations were performed by determining the alcoholic, phenolic, flavanoid contents along with reducing
sugar determination. In vitro antioxidant activity was performed by FTC, FRAP and TBA method. Validation experiments of
new Laportea arishta were performed to demonstrate linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ
as per ICH guidelines.

Key words: Laportea interrupta, Arishtas, Antioxidant, Validation

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