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Sub-chronic oral propylene glycol administration sustained normal liver function and histology: Suitable alternative vehicle for hydrophobic test-compounds

Gracious Oluwamayowa Oke,Christian Eseigbe Imafidon,Ayofe Isiaka Adekunle.

Abstract
Background: An undocumented controversy exists on the suitability/unsuitability of propylene glycol as a solvent or vehicle for hydrophobic test-compounds in animal experimentation(s). This is partly due to paucity of evidence that aims to probe its histological effects beyond H&E assessment.
Objective: To determine the potential suitability/unsuitability of oral propylene glycol as a vehicle for hydrophobic test-compounds by assessing its histological and biochemical effects in female Wistar rats.
Methods: The study recruited ten (10) Wistar rats which were divided into 2 groups of 5 rats each as follows; Group 1 received distilled water (2 mL/kg, p.o.) for four consecutive weeks while group 2 received propylene glycol (2 mL/kg, p.o.) for the same period of time.
Results: Propylene glycol caused no alteration in liver function as indicated by the plasma levels of AST, ALT, ALP and hepatic total protein level, when both groups were compared (p > 0.05). No significant difference between both groups was also recorded in the oxidative stress status (GSH and CAT) of their liver homogenates. Histological examinations using H & E (general histoarchitecture) as well as special stains such as Gordon and Sweet (reticular fibre formation), Massonís trichrome (collagen fibres) and PAS (glycogen content) did not reveal any feature of pathology. Quantification analyses of their liver sections, using image J, further revealed no significant difference (p > 0.05) in their histoarchitectural features.
Conclusion: Propylene glycol is a suitable alternative vehicle for hydrophobic test-compounds in experimental studies, as its sub-chronic administration sustained normal liver function and histology.

Key words: Distilled water; propylene glycol; hydrophobic test-compounds; liver function; Wistar rats



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