Home|Journals|Articles by Year|Audio Abstracts

Original Article

In vitro and In silico Antigout arthritic activities of Ethanolic and Aqueous stem extracts of Cissus quadrangularis - A TLR2 and TLR4 Receptor approach

Tharini Palani, Krishnan Shobha, Thirunavukkarasu P, Rajeswary Hari.


The in vitro anti-gout arthritic activity of ethanolic and aqueous extracts of Cissus quadrangularis stem was evaluated in terms of inhibition of xanthine oxidase, proteinase enzyme, protein denaturation and membrane stabilization. The Monosodium Urate (MSU) up-regulated expression patterns of toll like receptors namely TLR-2and TLR-4 were analyzed. The molecular docking was performed to select the antagonistic phyto ligand for receptors since their activation leads to the destructive immunological reactions occurring in gouty arthritis. The in vitro anti-gout arthritic activities were estimated using standard protocols. The MSU crystal induced toll like receptors gene expression was analyzed. Molecular docking and LIGPLOT analysis was performed with the GCMS derived phyto constituents in the ethanolic extract of Cissus quadrangularis with the receptors to find the interactions. Colchicine was used as a positive drug in the study. Among the two extracts, ethanolic extract revealed better in-vitro anti arthritic activity in the present investigation. Out of the GCMS derived 13 phyto components, based on docking score three main components namely Pentadecanoic acid, 14-methyl,-methyl ester,10-Octadecenoic acid, methyl ester, 4-one,2-(3,4-dihydroxyphenyl)-2,3-dihydro-3,5,7-trihydroxy showed better antagonistic action in the LIGPLOT analysis. It may be concluded these three ligands may further developed as a potential anti gouty arthritic drug

Key words: Anti gout, Monosodium urate, Toll like receptor, Xanthine oxidase

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.